RAPD-PCR analysis and gene expression of CYP450 in Tribolium castaneum adults in response to different insecticides

Document Type : Original Article

Authors

1 zoology department,faculty of science, Menoufia university

2 Entomology department, faculty of science, benha University

Abstract

Random amplified polymorphic DNA analysis by polymerase chain reaction (RAPD-PCR) was used as a tool to screen genetic variation in Tribolium castaneum adults treated by LC50 of Bacillus thuringienisis, Beauveria bassiana, teflubenzuron and spinetoram using ten random oligonucleotide primers for amplification. The results of cluster analysis using Dice coefficient among treated and untreated T. castaneum adults revealed that adult beetles were grouped into 2 main clusters. One contains control adults. The other cluster was divided into 2 main subclusters; one includes adults treated with LC50 of B. thuringienisis and teflubenzuron while the other subcluster contains adult beetles treated with LC50 of B. bassiana and spinetoram. Furthermore, the transcriptional response of Cytochrome P450 gene to each of the used insecticides in T. castaneum was examined by qRT-PCR. The results revealed that CYP450 was up-regulated by all the four insecticides tested. The highest level of up-regulation was recorded in adult beetles treated with LC50 of teflubenzuron (25.9-fold) followed by B. thuringienisis treated adults (7.6- fold), and spinetoram (6.8-fold). The least level of up-regulation was recorded in adults exposed to LC50 of B. bassiana (2.9- fold) as compared to control group.

Keywords

Catrina: The International Journal of Environmental Sciences
Volume 19, Issue 1
September 2019
Pages 19-27

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