Document Type : Original Article
Authors
1
Basic Science Department, Faculty of Oral and Dental Medicine, Nahda University, Beni-Suef, Egypt.
2
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
3
Genetics Department, Faculty of Agriculture, Beni-Suef University, Beni-Suef, Egypt
4
Biochemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
5
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia
Abstract
The growing demand for efficient microbial cellulase for lignocellulosic biomass degradation in bioenergy, food, and agricultural industries has driven exploration of actinomycetes from rhizosphere environments as novel enzyme sources. In this study, actinomycetes isolated from wheat rhizosphere soils led to the identification of Streptomyces acrimycini BUS-WH31, a strain demonstrating high cellulolytic activity and exceptional salt tolerance. Taxonomic characterization through 16S rRNA gene sequencing, morphological analysis, and chemotaxonomic profiling confirmed its taxonomic identity. Whole-genome sequencing revealed a linear chromosome of 4,936,986 base pairs with a GC content of 78.77%, enriched with genes involved in carbohydrate metabolism, antibiotic biosynthesis, and transport systems. Under optimized fermentation conditions, the strain produced thermostable cellulase enzymes with optimal activity at 60°C and pH 8.5, maintaining enhanced enzymatic activity in the presence of up to 8% NaCl. Additional enzymatic assays confirmed amylase, lipase, and protease production, underscoring its multifunctional biocatalytic potential. These findings position S. acrimycini BUS-WH31 as a robust candidate for industrial biomass conversion processes, particularly in saline environments, with genomic insights paving the way for targeted biotechnological applications in biofuel production.
Keywords
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