Comparison of Various Bioactive Compounds in Leaves and Seeds of Tribulus longipetalus Viv.

A study was conducted to analyze different bioactive compounds in Leaves and seeds of Tribulus
longipetalus include saponins, total proteins, amino acids, fat and flavonoids with one and two
dimensional, thin layer and column chromatography followed by spectrophotometric analysis. Results
indicate that leaves contained high concentrations of flavonoids and fat. Whereas levels of saponins,
proteins, amino acids, total minerals and other organic compounds were high in seeds. The analysis of
leaves and seeds of Tribulus longipetalus for these valuable organic compounds will provide important
raw materials that can be used for preparation of medicines in pharmaceuticals companies. A single
subcutaneous dose of CCl4 (5.93ml kg*1b.wt. which represents 24h LD100) severed as a toxicant, was
used in this study. The results showed that ethyl acetate fraction was the most active fraction, then
butanol, aqueous and dichloromethane fraction. By subjecting the ethyl acetate fraction to column
chromatography it afforded fractions which are effective in reducing CCl4 induced mortalities. The
efficiency of the isolated glycosides in reducing CCl4 –induced mortalities and some disturbances in
biochemical parameters (liver transaminases AST, ALT and creatine kinas CK activities, serum urea
and serum creatinine) were investigated in adult female Bufo regularis (Egyptian toads). The
biochemical studies confirmed the bioassay studies. This suggestes that these saponin glycosides may
be promoting in modulating CCl4- induced lethality and most of its toxic effects.