Department of Botany, University of Fort Hare, Alice, 5700, South Africa
Abstract
Pelargonium sidoides is a medicinal plant harvested for local and export trades in South Africa. In order
to contribute to the conservation and propagation initiatives of the herb, a study on the micropropagation
of the plant was conducted. Our findings revealed that callus induction was achieved by using different
concentrations of IBA and 2,4-D. Optimal response was obtained at 3 mg l-1 IBA under continuous dark
condition. The study also showed that shoot differentiation is achievable from stem derived callus after
one week in hormone free media under continuous light. The percentage of callus formation ranged from
0 to 88.9% depending on the type of auxin used, the concentration and photogenic conditions.
Transferred plantlets by simple acclimatization procedure survived and produced healthy plants. The
significance of these on the propagation and conservation of P. sidoides is highlighted in the discussion.
Lewu, F., Grierson, D., & Afolayan, A. (2007). Micropropagation of Pelargonium sidoides. Catrina: The International Journal of Environmental Sciences, 2(1), 77-81.
MLA
Francis Lewu; Don Grierson; Anthony Afolayan. "Micropropagation of Pelargonium sidoides". Catrina: The International Journal of Environmental Sciences, 2, 1, 2007, 77-81.
HARVARD
Lewu, F., Grierson, D., Afolayan, A. (2007). 'Micropropagation of Pelargonium sidoides', Catrina: The International Journal of Environmental Sciences, 2(1), pp. 77-81.
VANCOUVER
Lewu, F., Grierson, D., Afolayan, A. Micropropagation of Pelargonium sidoides. Catrina: The International Journal of Environmental Sciences, 2007; 2(1): 77-81.
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