Effect of Carbon Sources and Microelement Concentrations on in vitro Proliferation and Rooting of Pineapple (Ananas comosus)

Document Type : Original Article

Author

Department of Fruit Research, National Research Center, Dokki, Giza, Egypt

Abstract

The regenerated shoots of Pineapple (Ananas comosus Cv. Smooth Coyenne) during the establishment
stage were cultured individually on MS medium supplemented with BAP (2.0 mg/L) during the
proliferation stage and IBA (2.0 mg) during the rooting stage. Various sugars (glucose, fructose and
sucrose) and concentrations (0, 10, 20 and 30 mg/L) were tested. Copper and Boron microelements
concentrations were also tested. Data indicated that all sucrose treatments enhanced the proliferation and
rooting, however Glucose and fructose improved shoot length. Increasing copper sulfate to (0.05 mg/L)
produced the best proliferation. Meanwhile, Boric acid at the rate of 12.4 mg/L was the most suitable
concentration for improving rooting percentage.

Keywords