In vitro Culture of Ruta graveolens L.

Attalla, Hamdy; Abo El Soud, Islam; Eid, Manal H.

In vitro Culture of Ruta graveolens L.

Document Type : Original Article

Authors

¹ Faculty of Agriculture, Suez Canal University. Ismailia

² Horticulture Department, Faculty of Agriculture, Suez Canal University 41522 Ismailia, Egypt

³ Agricultural Botany Department, Faculty of Agriculture, Suez Canal University 41522 Ismailia, Egypt

Abstract

Shoot multiplication for Ruta graveolens L. was achieved when shoot tips excised from mature plants
and cultured on Murashige and Skoog (MS) medium containing BA at 0.5, 1.0 and 2.0 mg l-1 combined
with NAA at 0.1, 0.2 and 0.3 mg l-1. Highest rate of shoot proliferation was obtained after 8 weeks with
the medium supplemented with BA at 0.5 mg l-1 and 0.3 mg l-1 NAA. Complete plants with strong,
fibrous roots were obtained after transferring the separated shoots into rooting medium (1/2 MS or MS
media with or without NAA). The survival rate of transplants during acclimatization reached 100% and
the plants grew normally under the greenhouse conditions.

Keywords