Diversity of Four Lupinus L. Taxa Based on Seed Protein Electrophoresis and Amino Acid Profile

Document Type : Original Article

Authors

1 Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt

2 Botany Department, Faculty of Science, Alexandria University, Alexandria, Egypt

Abstract

The aim of the present study is to evaluate diversity among three Lupinus species (Lupinus albus L.,
Lupinus angustifolius L., and Lupinus digitatus Forssk.) using seed protein electrophoresis and amino
acid composition in order to elucidate the taxonomic relationships among them. In addition, to compare
the L. albus var. albus with closely related wild relatives in a trial to attain information useful for
breeding programs. The overall pattern of seed proteins and amino acid composition within the same
species revealed that there is a considerable variation along with the geographical region. The percentage
of polymorphism for all the studied taxa was 26% and a total of 18 bands are detected within the studied
Lupinus taxa, which are characterized by 4 common bands at 38, 36, 20 and 14.4 KDa. The dendrogram
constructed from different concentrations of amino acid in the studied Lupinus species, demonstrated that
the four studied taxa are not fully discriminated even at both different similarity coefficients and different
types of sorting. By using seed protein electrophoresis, the L. albus var. albus (L. albus cultivar) is
assembled with the wild L. albus below the same group. But at the same time the cultivar showed
considerable degree of variability from the wild species and separated alone by using both data from seed
proteins and amino acid compositions. The cultivar is characterized with both low degree of
polymorphism and deficiency in arginine, leucine and methionine which are considered from the vital
important amino acids. Contrary, both L. albus and L. angustifolius have considerable amounts of these
amino acids.

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