Activation tagging in aspen using a heat-shock inducible two component Ac/Ds-enhancer element system

Document Type : Original Article

Authors

1 Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

2 Johann Heinrich von Thuenen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf, Germany

Abstract

From our earlier work on the genetic transfer of the maize transposable element Ac and its functional
analysis in hybrid and pure aspen lines, it was shown that this element is active in transgenic aspen.
However, a two element transposon tagging system where the Ds element and the transposase gene are
put together in crosses is not feasible in trees due to the in part long vegetative phases. To overcome
this barrier, an inducible two element HSP-transposase /ATDs element system is suggested to induce
activation tagged variants following two independent transformation steps. The ATDs element is
comprised by a 35S enhancer tetramer and outwards facing two CaMV 35S promoter located near both
ends of the ATDs element. Following transposition of the ATDs element expression of genes can be
elevated which are located adjacent to the new integration site of the element.

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