Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System

Document Type : Original Article

Authors

1 Faculty of Agriculture, Suez Canal University. Ismailia

2 Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

3 Johann Heinrich von Thuenen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf, Germany

Abstract

Based on the Ac/Ds two element transposition system from maize an activation tagging approach was
suggested for the hybrid aspen (Populus tremula x tremuloides) line ‘Esch5’. A glucocorticoidinducible
two element Ac/ATDs element system was used to induce activation tagged variants
following two independent transformation steps. In combination with a 35S enhancer tetramer and
outward facing two CaMV 35S promoter located near both ends of the ATDs element, expression of
genes can be elevated located adjacent to the new integration site of the element. As selective marker
for ATDs transposition, knocking-out the expression of a phenotypic marker (rolC gene) was
considered.

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