Authors
1
Plant Production Department, Faculty of Environmental Agriculture sciences, Suez Canal University, El-Arish, Egypt
2
Plant Genetic Resources Department., Desert Research Center (DRC), Cairo, Egypt
3
Egyptian Desert Gene Bank, Plant Genetic Resources Department, DRC, El-Sheikh Zewied, North Sinai, Egypt
Abstract
Genetic relationships between six varieties and their fifteen hybrids of faba beans grown under the
natural environmental condition at North Sinai Research Station (NSRS) were studied using ISSR
molecular markers. Fifteen mer specific DNA primers were used. Out of used six arbitrary 15 mer
primers, five were identified and detected polymorphism. The results indicated that there were some
variations in banding patterns among these 6 parents which appeared a High level of polymorphism
between it. The results of ISSR –PCR profiles of the studied faba bean genotypes for 15 F1 hybrids
showed a High level of monomorphism was observed. The highest polymorphism with primer HB8
(GA) 6 GG at molecular weight 1100 bp. While the highest monomorphism with primers 17899B (CA)
6 GG at molecular weights (455, 749, 847 and1020 bp) bp, and HB9 (GT) 6 GG at molecular weights
(500, 680, 1021 and 1299) bp. The genetic relationships based on ISSR markers were developed using
SPSS computer program. Moreover, these molecular markers are useful tools for the breeder to decrese
the time of breeding program. The results of genetic relationships showed that the genotypes were
divided into two groups. Each group consists of the parents and their hybrids. The first group consist of
the parents (Nubaria1 and Sakha2) and eleven hybrids of Vicia faba (L1, L2, L3, L4, L5, L6, L7, L8, L9,
L10 and L11), while the second cluster included the rest of parents (Sakha1, Giza429, Giza716 and
Giza2) and others hybrids (L12, L13, L14 and L15). Passed on the later the obtained results from ISSR
analysis and the results of Dendrograms demonstrated the relationships refers to the cross direction in
breeding program was done correctly. For this reason, results of positive and negative molecular size
(pb) against different primers indicated that ISSR markers are effective in breeding programs for drought
tolerance.
Keywords
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