Evaluation of chlorophyll meter method in Egyptian wheat cultivars after Photobiostimulation with Red Polarized Light

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt

2 Integrated Control Research Department, Plant Pathology Research Institute, Agricultural Research Centre, Giza, Egypt

Abstract

The present study has been devoted to compare between the commonly used spectrophotometric method (SPM) for chlorophyll measurement and the portable chlorophyll meter (AtLeaf) method (PCM) for direct reading. Photobiostimulation of chlorophyll has been induced in leaves (30-day-old) of six cultivars of common wheat (Triticum aestivum L.) in Egypt, through pre-sowing seed treatments with 9 different doses (exposure times from 1- 1200 seconds) of monochromatic red polarized light (wavelength 632.8 nm and power intensity 0.5 mW.cm-2). The results obtained indicated that polarized light induced a significant increase in chlorophyll contents mostly in all cultivars, as compared to corresponding controls. As a general trend, low intensity red polarized light enhanced the chlorophyll content in leaves of the 6 tested wheat cultivars under study. Both the SPM and the PCM methods were efficient for chlorophyll assessment and the readings of the portable method showed significantly strong correlation with those of the spectrophotometric method. Thus, it might be concluded that easy to use and time rescuing portable chlorophyll meter method can be used efficiently in monitoring the chlorophyll contents of leaves of the 6 tested common wheat cultivars.

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