Egyptian Society for Environmental SciencesCatrina: The International Journal of Environmental Sciences1687-50523320081101Molecular Identification of Egyptian Eggplant Cultivars (Solanum melongena L.) Using RAPD and RFLP of 18S rRNA Gene Markers11018534ENHeshamAbdel-RazzakVegetable Crops Department, Faculty of Agriculture, Alexandria University, Alexandria, EgyptSamiaAbdAl-AzizNucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for
Scientific Research and Technology Application, Alexandria, EgyptElsayedHafezArid Lands Research Institute, Plant Molecular Pathology Department, Borg El Arab City, Alexandria, EgyptJournal Article20181107Seven Eggplant (Solanum melongena L.) genotypes, representing the three common Egyptian local
cultivars, were subjected to random amplified polymorphic DNA (RAPD) analysis using ten random
primers. The ten primers generated 657 RAPD fragments, of which 77 were polymorphic with 11.72 %.
The seven genotypes were discriminated with 3-11 distinct polymorphic bands generated from the
primers CHI 15 (3), and RAP, EZ and NAH (11). The genetic similarity was estimated for each primer
among the seven genotypes. Results of a cluster analysis showed that the genetic similarity between the
seven genotypes ranged from 58.0% to 92.0%. The highest genetic similarity of 92.0% was detected
between genotypes LB1 and LB2 (original population "OP" and selected population "SP"; long slender
black fruits), followed by 86.0% between genotypes RB1 and RB2 (OP and SP; round or egg shaped
black fruits), and 80.0% between genotypes LB2 (SP; long slender black fruits) and RB2 (SP; round or
egg shaped black fruits). However, the lowest genetic similarity of 58.0% was detected between both
genotypes LW1 and LW2 (OP and SP; long slender white fruits) and genotype PO (SP; purple oblong
shape fruits), followed by RB1 (OP; round or egg shaped black fruits) and PO (61.0%). Restriction
fragment length polymorphism (RFLP) of 18S rRNA gene was carried out using four different restriction
enzymes viz. EcoRI, TaqI, PstI and HindIII. Only the restriction pattern of Taq I enzyme showed distinct
polymorphic bands among the seven examined eggplant genotypes and divided them into three different
groups. However, the RAPD-PCR succeeded to divide them into two major groups; group one contained
only genotype PO (SP; purple oblong shape fruits) and the second group included the other genotypes.https://cat.journals.ekb.eg/article_18534_dd4536bcbc50fcb9686346af336e4e21.pdfEgyptian Society for Environmental SciencesCatrina: The International Journal of Environmental Sciences1687-50523320081101Effect of Drought Stress on Photosynthetic Efficiency of Glycine max L. Plants111618535ENShrifaAbu-MuriefahBotany Department (Science Section), Girls College of Education,
P.O. Box 27104, Riyadh 11417, Saudi ArabiaJournal Article20181107The current study is carried out to investigate the response of photosynthetic efficiency of soybean plant
to drought stress extended for six days. Plants exposed to water deficit, growth inhibition and chlorophyll
content losses increased. All drought treatments resulted in distinct physiological and biochemical effects
on photosynthesis in a duration dependent manner. Relatively high inhibitory effect was observed
throughout the experimental period, six days drought almost totally crushed the dynamics of chlorophyll
accumulation in soybean leaves. A marked decrease in the ratio of chlorophyll a/b occurred in 6 days
drought treated soybean leaves. Thus, a significant reduction in content of aminolaevulinic acid (ALA)
was observed after 6 days drought treatment compared with relatively less decrease after 2 and 4 days
drought treatments. The maximal photochemical efficiency of soybean leaves subjected to drought
treatment, decreased sharply in Fv/Fm values to approximately half of the corresponding control at the
end of drought duration. The photosynthetic strength of the plant indicated by Fv/F0 values showed that,
soybean leaves exposed to drought were significantly decreased especially at the end of experimental
period. Drought stressed soybean leaves showed a highly significant increase in H2O2 and MDA contents
reached about 4.5 fold increase relative to control after 6 days drought treatment. This means that drought
caused depression of photosynthetic efficiency of soybean leaves in a period-dependent manner.https://cat.journals.ekb.eg/article_18535_c98803f857cf5b6ac5fa34ecc8b7face.pdfEgyptian Society for Environmental SciencesCatrina: The International Journal of Environmental Sciences1687-50523320081101Treatment of Phenolic Industrial Wastewater Using Activated Sludge Process Preceded by Anoxic Selector172318537ENAbd El-GhanyAbulnourChemical Engineering and Pilot Plant Department, Engineering Research Division,
National Research Centre, Dokki, Cairo, EgyptHayamShaalanChemical Engineering and Pilot Plant Department, Engineering Research Division,
National Research Centre, Dokki, Cairo, EgyptTahaAbd El-RazekInstitute of Environmental Studies and Research, Ain Shams University, EgyptAliHassanInstitute of Environmental Studies and Research, Ain Shams University, EgyptSaharAliChemical Engineering and Pilot Plant Department, Engineering Research Division,
National Research Centre, Dokki, Cairo, EgyptJournal Article20181107Activated sludge process (ASP) has been widely used for the treatment of high organic load industrial
wastewater. Toxic compounds (e.g phenol at high concentration) limit the application of ASP. In the
present work, ASP preceded by anoxic selector was investigated for the treatment of phenolic effluents
generated from a chemical and pharmaceutical industry. The parameters affect the process (phenol
concentration, food to microorganism ratio (F/M), retention time and pH) were studied using batch and
completely mixed activated sludge (CMAS) type reactor (with and without anoxic selector). The results
showed that the maximum removal efficiency obtained by using batch process was 99.9% at 800 mg/l
initial phenol concentration, F/M ratio of 0.579 (g COD/g MLVSS.d) and pH around 7. For CMAS only,
the maximum applied phenol concentration was 2000 mg/l to yield effluent phenol concentration of 35
mg/l. For anoxic selector-CMAS, the maximum applied phenol concentration was 5500 mg/l to yield
effluent phenol concentration of 0.01 mg/l. It was found that the performance of industrial wastewater
treatment was significantly enhanced using anoxic selector. It is concluded that the use of properly
designed anoxic selector prior to CMAS significantly improves the performance of the process and can
tolerate much higher phenol concentration than that tolerated by the conventional CMAS. The
application of the proposed system contributes to the establishment of technically viable solutions for
water bodies pollution by phenolic wastewater.https://cat.journals.ekb.eg/article_18537_85cbdd023c01254311a341ea91b27a27.pdfEgyptian Society for Environmental SciencesCatrina: The International Journal of Environmental Sciences1687-50523320081101Micropropagation of Capparis cartilaginea Decne.253218539ENRaifaHassaneinBotany Department, Faculty of Sciences, Ain Shams University, Cairo, EgyptMadihaGabrEcology and Dry Land Agriculture Division, Desert Research Center, Cairo, EgyptAhmedAhmedEcology and Dry Land Agriculture Division, Desert Research Center, Cairo, EgyptGhadaHegaziEcology and Dry Land Agriculture Division, Desert Research Center, Cairo, EgyptJournal Article20181107Micropropagation was used to propagate the wild economic important plant; Capparis cartilaginea
which is difficult to propagate in normal conditions. For establishment and multiplication, MS medium
containing 0.1 mg/L NAA + 3 mg/L BA was the most suitable medium for Capparis cartilaginea shoot
tip explants. This medium gave the highest percentage of survival (100%), axillary shoot formation
(76.74%), mean number (3.2) and length (0.564 cm) of axillary shoots per explant. The concentrations of
0.5 and 1 mg 2iP/L were suitable for the elongation of one (3.82 cm) and three shoots (3.114 cm)
explants, respectively. The highest rooting percentage (22.22%) was obtained on half strength MS agar
gelled medium supplemented with NAA at 2 mg/L under six days of dark incubation. A comparative
study through the nucleic acids content and the protein banding pattern was carried out between the in
vitro produced plantlets and the mother plant in autumn and spring seasons. The concentrations of RNA
and DNA as well as the number of protein bands in the plantlets produced from tissue culture were
increased over those of the newly growing parts of the mother plant.https://cat.journals.ekb.eg/article_18539_47954b4c264f4bcb1ea3f5964767be4e.pdfEgyptian Society for Environmental SciencesCatrina: The International Journal of Environmental Sciences1687-50523320081101In vitro Culture of Ruta graveolens L.333618540ENHamdyAttallaFaculty of Agriculture, Suez Canal University. IsmailiaIslamAbo El SoudHorticulture Department, Faculty of Agriculture, Suez Canal University 41522 Ismailia, EgyptManal H.EidAgricultural Botany Department, Faculty of Agriculture, Suez Canal University 41522 Ismailia, EgyptJournal Article20181107Shoot multiplication for Ruta graveolens L. was achieved when shoot tips excised from mature plants
and cultured on Murashige and Skoog (MS) medium containing BA at 0.5, 1.0 and 2.0 mg l-1 combined
with NAA at 0.1, 0.2 and 0.3 mg l-1. Highest rate of shoot proliferation was obtained after 8 weeks with
the medium supplemented with BA at 0.5 mg l-1 and 0.3 mg l-1 NAA. Complete plants with strong,
fibrous roots were obtained after transferring the separated shoots into rooting medium (1/2 MS or MS
media with or without NAA). The survival rate of transplants during acclimatization reached 100% and
the plants grew normally under the greenhouse conditions.https://cat.journals.ekb.eg/article_18540_2c3a290468be441eefe31696301791d7.pdfEgyptian Society for Environmental SciencesCatrina: The International Journal of Environmental Sciences1687-50523320081101Floristic composition of Sahara Area in Libya375318541ENKhadejaBaayoBiology Department, Faculty of Science, Seventh of October University, Masratah, LibyaJournal Article20181107The present study aimed to analyze the floristic structure of Desert territory in Libya with focusing on the
distribution of global important species such as medicinal, endemic and rare species. Vegetation survey
was conducted in Al-Joufrah Oases (Hun, Wedan, Soknah, and Zilla). The results revealed that the
Libyan Desert area harbors 244 plant species, which belong to 164 genera and 53 families (108 annual
species and 136 perennial species). One hundred and twenty-seven species were recorded in Joufrah area
including twenty-four species as new records, 26 medicinal plant species, 11 endemic species, and 9 rare
species. The most abundant families were Poaceae (35 species), Fabaceae (27 species), Asteraceae (22
species), Brassicaceae (18 species), and Zygophyllaceae (16 species). Twenty-seven families were
represented by 2-9 species and twenty-two families were represented by only one species. The most
dominant life forms in the area were the annuals followed by the chamaephytes. Phytogeographically,
flora of Libya belongs mainly to Arabian Sahara followed by the Arabian Sahara+ Irano-turranean
Sahara, then the Arabian Sahara and Sudano-Zambesian.https://cat.journals.ekb.eg/article_18541_f0ebe9187acd58ae16701b93a9bf9cc2.pdfEgyptian Society for Environmental SciencesCatrina: The International Journal of Environmental Sciences1687-50523320081101Otolith Morphology and Body Size Relationships for Selected Fishes in Suez Canal and Gulf of Suez556318543ENMagdyAlwanyDepartment of Marine Science, Faculty of Science, Suez Canal University, Ismailia, EgyptElhamHassanDepartment of Marine Sciences, Faculty of Science, Suez Canal University, Ismailia, EgyptJournal Article20181107The morphology of the sacular otolith (sagitta) was studied by means of image analysis of 28 extant
species from Suez Canal and Gulf of Suez. The shape, margins and rostrum of sagittal otoliths for all
species were analyzed. No difference was detected between left and right otolith length for any of the
otolith pairs (paired t-tests, all p > 0.20). The largest otolith was recorded in Morone saxatilis and
Argyrosomus regius; while the smallest otolith was recorded in Myripristis botche. ◌ِAlso, otolith weight
or mass was varying between studied fishes, where the heaviest otolith was recorded in Plectropomus
leopardus. Morphological characteristics of fish otoliths were highly variable between species, ranging
from the relatively simple disc shape (Terapon puta, Terapontidae) to the irregular shape in Myripristis
botche (Holocentridae). The shapes of the otolith of studied species were varying from oblong, ovate,
fusiform, elliptic, rhomboidal and triangular. The margin sculpturing of these otoliths have four
characters: irregular, lobed, entire and dentate. The relationships between otolith and fish size which
examined by plotting the value of the otolith length and weight against the length and weight for each
fish.https://cat.journals.ekb.eg/article_18543_4579e1c5479d5d594f56ef96b0070ee0.pdfEgyptian Society for Environmental SciencesCatrina: The International Journal of Environmental Sciences1687-50523320081101Geo-Hazards Assessment of Western Adabyia Port Area, Gulf of Suez, Egypt657818544ENHamdyAboulelaMarine Science Department, Faculty of Science, Suez Canal University, 41522 Ismailia, EgyptMohamedArnousGeology Department, Faculty of Science, Suez Canal University, 41522 Ismailia, EgyptJournal Article20181107The aim of the present study is to assess the present geo-hazards, both flash flooding and seismic activity
at the western part of Adabiya Port area, Gulf of Suez. Assessment of include Remote Sensing technique
was applied using satellite images, topographical, geological and other geological data through GIS
technologies. GIS data integration, including the morphometric; local seismic activity and the structural
data analysis, indicated that the area is threatened by two types of geo-hazards. The morphometric
analysis strongly supported the high probability of flash flooding in different sites within the study area.
The structural lineaments, extracted from enhanced ETM+7 image, showed that the major of seismic
activity are related to the segments of the fault system of the Gulf of Suez and Gulf of Aqaba. The
seismic activity hazard was taken into consideration through identification of the sources of events. The
seismic activity in the study area markedly increases from north to south. The distribution of seismic
activity pattern and data analysis for the study area clearly exhibits the urgent need for an assessment and
rehabilitation program to mitigate geo-hazard along the existing structures.https://cat.journals.ekb.eg/article_18544_18a1c8654fe2bf0acffd70c89a97cc4f.pdf